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UPLC - Ultra Performance Liquid Chromatography Beginner's

Hochleistungsflüssigkeitschromatographie - Wikipedi

  1. In 2004, Waters launched the technique known as UltraPerformance LC (UPLC) based upon sub 2-micron porous particles and trademarked the acronym UPLC. As vendors entered the market with similar instruments UHPLC was coined as a way to refer to instruments similar to UPLC since UPLC is a Waters trademark
  2. Wofür steht UPLC im Text In Summe ist UPLC ein Akronym oder Abkürzungswort, das in einfacher Sprache definiert ist. Diese Seite zeigt, wie UPLC in Messaging- und Chat-Foren verwendet wird, zusätzlich zu Social-Networking-Software wie VK, Instagram, Whatsapp und Snapchat
  3. Der Begriff HPLC steht für High Performance Liquid Chromatography, während der Begriff UPLC für Ultra High Performance Liquid Chromatography steht. Daher ist die UPLC-Technik eine entwickelte HPLC-Version. Somit ist die Funktionsweise für beide Techniken gleich
  4. The term HPLC stands for High Performance Liquid Chromatography whereas the term UPLC stands for Ultra High Performance Liquid Chromatography. Therefore, the UPLC technique is a developed version of HPLC. Thus, the method of operation is the same for both techniques
  5. High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture
  6. Download Aarogya Setu Mobile App : E-tendering customization fees collection. In case of any inconvenience, you may kindly contact undersigned Dhiraj Kuma
  7. Als die erste Säule für die Methoden Entwicklung bietet Ihnen der 1.7 µm ACQUITY UPLC BEH C18 Sorbent die universellste Säulen Wahl für die UPLC Trennung. Die trifunktionell gebundenen BEH Partikel ermöglichen Ihnen eine
ACQUITY UPLC H-class | UPLC | Scanco Analytical Instruments

Ultra Performance Liquid Chromatography - an overview

  1. Chromatographic peaks in UHPLC are narrower and sample throughput is higher. However, there are other factors which must be considered. Based on this information the consumer might choose UHPLC because of the efficiency of the resulting separation. For the novice user, HPLC is a more robust, rugged methodology
  2. In 2004, Waters launched the technique known as UltraPerformance LC (UPLC) based upon sub 2-micron porous particles and trademarked the acronym UPLC. As vendors entered the market with similar..

UPLC operates at considerably high pressures of 15,000 psi (psi, in this case, stands for Pounds per square inch and is a unit of pressure), while HPLC operates at relatively lower pressures of 500-6000 psi. The key difference between HPLC and UPLC here is that UPLC has a clear advantage of HPLC, thanks to its higher pressure Ultra performance liquid chromatography™ (UPLC) takes advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control Ultra-performance liquid chromatography (UPLC) is a combination of a 1.7 μm reverse-phase packing material and a chromatographic system that can operate at pressures in the 6000-15 000 psi range (conventional HPLC uses 3-5 μm packing material and operates between 2000 and 4000 psi).There is a greater (S/N) due to the reduction in band broadening, and thus an increase in sensitivity Aufbau des quaternären Solvent Managers (QSM) der ACQUITY UPLC H-Class und des Sample Managers mit Durchflussnadel (SM-FTN) bieten die Flexibilität und den Bedienkomfort Ihrer jetzigen HPLC, erreichen aber die hocheffiziente Trennleistung, die nur die UPLC liefern kann. analytica-world.com. analytica-world.com

Diagnosetests mit Connections INSIGHT und der ACQUITY UPLC Konsole Die ACQUITY UPLC Konsole ist eine Software zum Betrieb der ACQUITY UPLC-Systemmodule, einschließlich des binären Solvent Managers. Es handelt sich um eine grafische Benutzeroberfläche, mit der in Echtzeit Informationen zum Systemstatus angezeigt werden können, das System direkt gesteuert werden kann, Fehler behoben sowie automatische Wartungs- funktionen durchgeführt werden können UPLC bedeutet Ultrahochmolekulares Druck flüssige Chromatographie. Wir sind stolz darauf, das Akronym UPLC in der größten Datenbank mit Abkürzungen und Akronymen aufzulisten. Die folgende Abbildung zeigt eine der Definitionen von UPLC in Englisch: Ultrahochmolekulares Druck flüssige Chromatographie. Sie können die Bilddatei herunterladen, um sie zu drucken oder an Ihre Freunde per E-Mail. Looking for online definition of UPLC or what UPLC stands for? UPLC is listed in the World's largest and most authoritative dictionary database of abbreviations and acronyms UPLC is listed in the World's largest and most authoritative dictionary database of abbreviations and acronym KNAUER stellt eine Reihe analytischer HPLC-Systeme und analytischer UHPLC-Systeme her, die Ihre Arbeit unterstützen und erleichtern

Video: ACQUITY UPLC System : Water

UPLC has filed Company application No. 17/2009 under section 542, 543 & 544 of the Companies Act, 1956 for the irregularities/acts of misfeasance of the Directors/ Ex-Directors of BPL/ BPLDDL and other officials, pending before Hon'ble Allahabad High Court. 170.00 Lakhs Equity Shares- Jedes Flüssigchromatographiesystem ist so konzipiert, dass Sie selbst komplexeste Proben zuverlässig trennen können. Das umfangreiche Portfolio an innovativen Thermo Scientific HPLC- und UHPLC-Systemen ermöglicht Ihnen die Auswahl des Systems, das Ihren Anforderungen am besten entspricht

The purpose can now be solved by the separation method called UPLC which is the modified HPLC method comprising high pressure and small sized particles (less than 2 µm) used in the column, so the length of the column decreases leading to time saving and reduction in the consumption of solvent. The underlying principle of UPLC is based on van Deemter statement which describes the connection. Ultra high performance liquid chromatography (UHPLC) and ultra performance liquid chromatography (UPLC) such as the Waters ACQUITY® APC™ are an advancement on traditional gel permeation chromatography (GPC) separations. Using advanced hybrid particles, which are smaller than in analytical GPC columns, ACQUITY APC columns offer improved resolutions, faster separation, reduced solvent use, and utilize low-dispersion (band broadening) hardware. A follow-on benefit is that the tall narrow. UPLC can be regarded as new invention for liquid chromatography. UPLC refers to Ultra Performance Liquid Chromatography. UPLC brings dramatic improvements in sensitivity, resolution and speed of analysis can be calculated. It has instrumentation that operates at high pressure than that used in HPLC & in this system uses fine particles(less than 2.5µm) & mobile phases at high linear velocities decreases the length of column, reduces solvent consumtion & saves time Die Ultra Performance Liquid Chromatography (UPLC) nutzt die Vorteile, die sich aus der Verwendung kleiner Partikel unter erhöhtem Arbeitsdruck ergeben. Sie ermöglicht chromatographische Trennungen mit hoher Auflösung, Empfindlichkeit und Geschwindigkeit. Soll die UPLC ihr volles Potenzial entfalten, muss u.a. die Technologie der Photodiodenarray-(PDA)-Detektoren Schritt halten

Further, ultra-high-performance chromatography (UPLC) instruments with UV detection are available, offering reduced analysis times, requiring lower sample and mobile phase volumes and delivering better resolution. We are experienced in performing reversed-phase chromatography on a large selection of columns from various vendors UPLC-MS/MS is still limited to conditions that are suitable for mass spectrometry operations. There are restrictions on pH, solvent choice, solvent additives and flow rates for UPLC to achieve optimal ESI-MS/MS sensitivity. For the chromatographic analysis and electrospray ionization of bepotastine and valsartan, we initially attempted to develop a reversed-phase chromatographic method. Proven systems that are rugged and versatile, with the flexibility to customize. Meet the needs and requirements for your application by selecting from our portfolio of Thermo Scientific UltiMate 3000 HPLC and UHPLC systems

What is the Difference Between UHPLC and UPLC

The 1290 Infinity II LC System is an ultrahigh performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. Find out more here Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range Recycle instrument power to restart the three-minute pump-down cycle on the degasser. It may take several cycles to purge the vacuum lines of excess solvent vapors. This will effectively purge degasser lines of excess solvent vapors and pull down to suitable vacuum The InfinityLab Analytical HPLC Systems are highly efficient HPLC and UHPLC systems that feature the latest technology. The 1220 Infinity II LC delivers high quality for an affordable price. The 1260 Infinity II LC is the flexible instrument choice, while the 1260 Infinity II Prime LC brings more operational convenience. The 1290 Infinity II LC embodies the next generation of liquid. Das HIC-ESP Ionenchromatographie-System von Shimadzu - profitieren Sie jetzt auch in der Ionenchromatographie von über 40 Jahren Erfahrung in HPLC Analytik. Das robuste und platzsparende System ist ausgestattet mit inerten Pumpen, die keine Reaktion mit der Probe eingehen und die durch eine besonders geringere Pulsation einen entscheidenden Vorteil für den druckempfindlichen.

This almost sounds like a line from Shakespeare, but HPLC or high-performance liquid chromatography and ultra-high-performance liquid chromatography (UHPLC) came a little after the 'Bard of Avon.' Both HPLC and UHPLC are common techniques in the analytical laboratory, but recent years have seen a shift from HPLC to UHPLC Ultra Performance Liquid Chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decrease of time and solvent consumption. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. Special analytical columns UPLC Acquity UPLC BEH C 18 packed.

The first ACQUITY UPLC injector was a loop-based design in which sample was drawn from a vial and subsequently transferred to a sample loop, which was then injected by switching the sample valve position from load to inject. This design offers two advantages: low dispersion and small dwell volume. In the direct injector design, popular in many HPLC systems, the sample needle becomes part of the sample loop, and the volume of the needle and sample loop combination is 2× larger than the. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. Special analytical columns UPLC Acquity UPLC BEH C(18) packed with 1.7 microm particles are used in connection with this system. The quality control analyses of four pharmaceutical formulations were transferred from HPLC to UPLC system. The results. ACQUITY UPLC I-Class PLUS-System Lösungen für komplexe Herausforderungen. Das ACQUITY UPLC I-Class PLUS-System eröffnet mit seinen branchenführenden Trennverfahren neue Möglichkeiten, mit denen Sie Auflösung und Empfindlichkeit Ihrer Verfahren maximieren können. Profitieren Sie außerdem von einer optimierten Charakterisierung Ihrer Proben und verbessern Sie die Produktivität Ihres Labors. Branchenführende, äußerst geringe Dispersion und sehr niedriges Totvolumen ermöglichen.

Waters ACQUITY UPLC System for sale | Complete Systems

Waters Acquity UPLC System is available from The Lab World Group. For more information, please visit us at https://www.thelabworldgroup.com/waters-acquity-up.. In UPLC, or ultra-high performance liquid chromatography, column particle size of less than 2um can be used. This allows for better separation than the typical particle size of 5um that are used in HPLC. It also allows for a very fast analysis. Though, UPLC is a trademark technology, it is used as a general term for this technique. Whereas the pump pressure in HPLC is 40MPa, in UPLC this. Definition : UPLC is a chromatographic technique that can separate a mixture of compounds, and is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture. Utilizing a smallpackaging particle sizes columns and ultra high pack pressure 9/23/2012 APPLICATIONS Drug Discovery -UPLC improves the drug discovery process by means of high throughput screening, combinational chemistry. Analysis of natural products and herbal traditional medicines. Analysis of Dosage form- It provides high speed, accuracy and reproducible results for analysis of drugs and their related substance. Thus method development time decrease

Trotz sorgfältigem Umgang mit stationärer und mobiler Phase sowie fachgerechter Wartung Ihrer HPLC-Anlage können immer wieder Probleme bei der HPLC-Analytik auftreten ANSWER. Please see the ACQUITY UPLC System Operator's Guide (page 119 onwards) for the recommendations.. Attached below are screen shots from the guide L'UPLC (Ultra Performance Liquid Chromatography) est une marque déposée de Waters corporation qui désigne certains de ses systèmes de chromatographie en phase liquide à haute pression. Introduite en 2004 et faisant appel aux mêmes principes que l'HPLC, l'UPLC améliore l'HPLC dans trois domaines : résolution chromatographique, vitesse d'analyse et sensibilité UHPLC has come a long way since Waters developed their own patented UPLC machines in 2004, with a lot of companies now developing their own UHPLC systems and columns. UHPLC provides a better resolution than traditional HPLC due to the shorter column length and smaller sub-2-micron particles in the columns, and UHPLC systems have specialized pumps that can deal with the higher pressures. Once. Title:UHPLC/Q-TOF-MS Technique: Introduction and Applications VOLUME: 12 ISSUE: 6 Author(s):Hamid Khan and Javed Ali Affiliation:JK College of Pharmacy, Bilaspur, CG, India-495001. Keywords:UHPLC/Q-TOF-MS, applications, identification, determination, pharmaceutical analysis. Abstract:The ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS.

Was bedeutet UPLC? -UPLC Definitionen Abkürzung Finde

UPLC:超高效液相色谱(Ultra Performance Liquid Chromatography)色谱理论认为提高色谱柱的效能(efficiency)就能增加仪器的解析度(resolution),而运用粒径低于2μm的小颗粒无疑是增加效能的好方法。但减小固定相的粒度以增加色谱柱效能一直的色谱仪器科学的瓶颈,因为小颗粒不仅要求系统能承受高于目前极限压力(比如9000psi),需要更小的系统体积(死体积),并且需要能. The PATROL UPLC process analyzer moves UPLC analysis from off-line quality control laboratories directly to the manufacturing process, resulting in significant improvements in production efficiency. The technology strategy is clearly focused on accelerating industry adoption of UPLC technology through a diverse range of systems based on a common, proven platform

Demonstrates the correct procedure for removing the needle assembly in the ACQUITY UPLC Sample Manager Echte UPLC-Performance setzt voraus, dass die Systemdispersion (d. h. die außerhalb der Säule verursachte Peakverbreiterung) den Peakbreiten entspricht, die mit Partikeln einer Größe von weniger als 2 μm assoziiert werden. Auch wenn andere Systeme auf hohen Druck ausgelegt sind, erzielen sie nicht notwendigerweise so spitze Peaks wie UPLC-Performance und das ACQUITY UPLC H-Class PLUS. UPLC shows a dramatic enhancement in speed, resolution as well as the sensitivity of analysis by using particle size less than 2 pm and the system is operational at higher pressure, while the.

Estos nuevos aparatos, denominados ultra performance liquid chromatography (UPLC) pueden trabajar con valores de hasta 100 MPa de presión (unas 1000 atmósferas). (Hay que tener en cuenta que las siglas UPLC son una marca registrada de Waters Corporation aunque a veces se utilizan de forma general para designar este tipo de aparatos) UPLC:超高效液相色谱(Ultra Performance Liquid Chromatography,UPLC)色谱理论认为提高色谱柱的效能(efficiency)就能增加仪器的解析度(resolution),而运用粒径低于2μm的小颗粒无疑是增加效能的好方法。但减小固定相的粒度以增加色谱柱效能一直的色谱仪器科学的瓶颈,因为小颗粒不仅要求系统能承受. Your Lab. Your LC. What's the right quaternary LC for your analysis? Let's talk about finding the LC that's right for your lab

uplc是超高效液相色谱,主要用来分离,配的也有紫外检测器;ms/ms,可以做二级质谱的质谱仪,二级是可以把一级的碎片离子. Our UPLC Universal Power-Line Carrier is frequency programmable across the entire power-line carrier spectrum without changing any hardware components UPLC™ 4.5X Speed 2X Sensitivity 1.5X Resolution 1 10 X m u l t i p l i c a t i o n f a c t o r UPLC M a ximum Sp eed Same Resolution H P LC UPLC Incr ea sed Sp eed M a ximum Resolution 2. 1 x 5 0 mm ACQUITY UPLC C olumn 2. 1 x 100 mm A CQUITY UPLC C olumn Resolution Sensitivity Sp eed The differences in the speed, sensitivity and resolution. UPLC advocates for prisoners, tenants, and disabled people denied public benefits. Tenants' Rights. We assist clients in fighting illegal evictions, recovering security deposits, and working with tenants whose landlord has allowed the building to fall into disrepair. Prisoners' Rights. We have multiple class action lawsuits currently filed against the state of Illinois for unconstitutional. For over 30 years scientists have been using analytical flow LC to develop drugs, monitor food, check water safety, diagnose diseases, and test for drug abuse. The ExionLC system supports multiple components and configurations so your LC can adapt to the changing demands of your laboratory while maximizing throughput, ultimately reducing your cost per sample

Unterschied zwischen HPLC und UPLC / Analytische Chemie

Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube UPLC: Ultra Performance Liquid Chromatography: UPLC: Ultrahigh Pressure Liquid Chromatography (chemistry) UPLC: United Power Line Council: UPLC: Unauthorized Practice of Law Committee (various locations) UPLC: University of the Philippines Law Center: UPLC: Uttar Pradesh Loktantrik Congress (Indian political party

Difference Between HPLC and UPLC Compare the Difference

UPLC® 시스템은 분석에 있어 우수한 데이터를 제공할 뿐만 아니라 분석시간과 비용을 감소시켜 줍니다. UPLC는 직선범위(linear range) 유속... ACQUITY UPLC | 중부대학교 산학협력단 | 2010.11.1 Agilent Technologies zählt zu den führenden Anbietern von HPLC-Anlagen und HPLC-Säulen. Der Analytics-Shop bietet mehr als 3.000 HPLC-Säulen, wie die Zorbax Eclipse oder Zorbax SB (Stable Bond) von Agilent an:. Phase: Zorbax, Poroshell, Polaris, Pursuit, ChromSpher etc. Packungsspezifizierung: Amino, C18, C8, CN, Diphenyl, Phenyl, Silica, etc.. 高效液相層析(英語: high performance liquid chromatography ,縮寫 HPLC),又譯高效液相色譜法,以前曾指高壓液相層析法(high pressure liquid chromatography),是一種色譜分析技術,用來分離混合物,以確認並量化各個成分的比例。 它依賴泵加壓樣品以令其通過填充有吸附劑的壓力柱,導致樣品的各個成分. For users of ACQUITY UPLC and UHPLC instruments, systems and columns. « 1 2 3 4 5 6 7 25

Waters ACQUITY UPLC H-Class Bio System

High-performance liquid chromatography - Wikipedi

The ACQUITY UPLC H-Class is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flow-through-needle injector to deliver the advanced performance expected of UPLC type separations - high resolution, sensitivity and improved throughput - while maintaining the robustness and reliability that ACQUITY systems are known for. Choosing the. Dabei liegt unser Schwerpunkt auf HPLC, UHPLC/UPLC und Massenspektrometrie. Für einen reibungslosen Ablauf senden Sie uns vorab eine Liste der Geräte, die Sie gerne prüfen lassen wollen sowie die verwendete Software. Sofern Sie spezielle Prüfvorschriften haben legen Sie diese auch bei. Zusammen finden wir dann einen passenden Termin

U.P.Electronics Corporation Limite

Große Auswahl an HPLC-Säulen / hohe Reproduzierbarkeit / für Methodenentwicklung / sehr hohe Effizienz / auch für Retention polarer Verbindungen geeigne The ACQUITY UPLC/UV Qualification Standards Kit is intended for performing system-level performance qualification on an ACQUITY System with TUV or PDA detectors, with or without an ELS detector in line, using the standard 10-mm flow cell configuration. This kit is designed for use by a Waters certified engineer or user as part of a performance maintenance or qualification activity Ultra Performance Liquid Chromatography (UPLC) Ultra performance liquid chromatography (UPLC) analysis. For the speciality chemical and pharmaceutical industries, it is more important than ever to work efficiently, rapidly and to the highest quality levels. Ultra Performance Liquid Chromatography (UPLC) brings faster, more rapid method development and validation, more efficient LC quantitative analysis, and high-throughput QC analysis HPLC and UPLC are both Liquid Chromatography used in separating the components of a compound or mixture. HPLC stands for High Performance Liquid Chromatography, and is a technique used to separate different constituents of a compound using high pressure to push solvents through the column. It is the most widely used technique to identify, quantify and separate components of a mixture. HPLC is widely used for the analysis of constituents of a pharmaceutical actives, drug products, pesticides. In order to improve separation efficiency and peak resolution, ultra performance liquid chromatography (UPLC) can be used instead of HPLC. This LC variant uses columns packed with smaller silica particles (~1.7 μm diameter) and requires higher operating pressures in the range of 310.000 to 775.000 torr (6000 to 15000 psi)

186002350 - UPLC-Säule ACQUITY BEH C18 1,7µm 2,1x50mm Col

For demanding applications when you need to collect more information in less time, our Flexar UHPLC is the perfect solution. This UHPLC is designed for a high productivity environment and delivers high sensitivity and resolution, exceptional flow accuracy and precision and faster results « HPLC Calculator » is a tool developed at the LCAP to improve your HPLC analytical methods in terms of time and/or resolution. It is not an optimization software and cannot find the mobile phase conditions necessary to separate compounds of interest. Only kinetic parameters are evaluated.1. The calculator determines chromatographic performances (i.e. efficiencies and induced backpressures. Sales and support of analytical instrumentation, Service and maintenance of standard- and dedicated setups, Customs tailored solutions for hard- and software, Application support, Training courses, Validation and certificatio S&U very encouraged by latest trading update, confident economy will rebound next year. S&U PLC's (LON:SUS) Anthony Coombs talks to Proactive London's Katie Pilbeam about the financing company's latest trading update which he is very encouraged by These indicate that the vasodilation of EEJ is in part related to causing the release of nitric oxide, activation of K(+) channels, inhibition of influx of excalcium, and release of calcium from sarcoplasmic reticulum. A total of 20 main ingredients, were identified in EEJ by UPLC-DAD/Q-TOF-MS. The vasodilation activity should be attributed to the high content of flavonoid glycosides and iridoid glycosides found in EEJ

HPLC vs UHPLC - Learn the difference HPLC vs UPLC

Our UPLC-II power-line carrier is the most economical medium of protective relay communications Dr. Jürgen Maier-Rosenkranz, Spezialist für nahezu alle Bereiche der Flüssigkeitschromatographie und deren Anwendung unter GLP oder ISO9000:200 Used Waters UPLC Waters is a leading manufacturer of lab and analytical equipment, specializing in liquid chromatography, mass spectrometry, and thermal analysis. The Waters Acquity UPLC H-Class System is the first UPLC that offers excellent performance and quaternary solvent blending The H-Class and ACQUITY, differ in the dwell volume of the system, the H-Class has the larger volume and thus the ACQUITY UPLC system has a faster injector cycle time and less of an equilibration time, it is better for ballistic (short and steep) gradients, but the run times have to be very short <2 min for you to notice. The H-Class injector, is slower, but not by much, and only requires a single needlewash and there are no loops to change. It's a matter of what' is best for you. Although.

Waters Acquity UPLC H-Class system (PDA) | eBayBEH (Ethylene Bridged Hybrid) Technology : Waters

Government orders for E-Procurement in Mining. 751/86-2017-57(स)/2017; Government orders for E-Tendering. 1107/78-2-2017-42आई०टी० /201 The Waters ACQUITY UPLC H-Class System combines chromatographic performance of UPLC with the flexibility of quaternary solvent blending. Designed to accommodate both UPLC and legacy HPLC assays, existing HPLC methods may be run as-is or transferred to full UPLC separations on the same system. The flexibility of multiple column selection (up to six), integrated solvent selection valve and software features such as Auto·Blend Plus™ (for on-line pH blending and/or control of ionic strength. ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these new chemistries also utilize new, proprietary endcapping.

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